ProductCategory:Pre-maderecombinantAAV;AAV-CAG-GCaMP6s;AAV-CBA-GCaMP6s;AAV9-CAG-GCaMP6s;AAV9-CBA-GCaMP6s
Description:
GCaMPisageneticallyencodedcalciumindicator(GECI)andcreatedfromafusionofgreenfluorescentprotein(GFP),calmodulin(CaM),andM13,apeptidesequencefrommyosinlightchainkinase. WhenGECIbindsCa2+,theconformationalchangeofGCaMP inducesproximityofthe GFPparts,eliciting strongGFPfluorescencesignal,whichallowsmeasuringactionpotentialsandotherreceptoractivationeventsthattriggerCa2+influx.TheadvantageofGECI"sarethattheycanbegeneticallyspecifiedforstudiesinlivingorganisms.
GCaMP6isanovelultra-sensitiveformatofGCaMPthatoutperformedothersensorsintermsofaccuracyandsensitivityinmeasuringcytosolCa2+level. MutationsincalmodulinpartofGCaMP6createdseveralvariants whichshoweddifferentcalciumfluorescencedecayrate(fast- GCamP6f, slow -GCaMP6s andmedium-GCaMP6m).
ThisAAV9-CAG-GCaMP6sisapre-packagedrAAVinSEROtype9(withcapsidfromAAVserotype9and2xITRfromAAVserotype2)whichover-expressesgeneticallyencodedcalciumindicatorGCaMP6s underCAGpromoter(alsoknownasCBApromoter).
Titer: >1x1013 VG/mL
Size: >30 µL(StdPack)
StorageBuffer: PBS
InfectionProtocol: 
VectorMap: 
